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KMID : 0545120050150020337
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Journal of Microbiology and Biotechnology
2005 Volume.15 No. 2 p.337 ~ p.345
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Degradation of Phenanthrene by Bacterial Strains Isolated from Soil in Oil Refinery Fields in Korea
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Kim JD
Shim SH/Lee CG
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Abstract
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The degradation of phenanthrene a model PAH compound by microorganisms either in the mixed culture or individual strain isolated from oil-contaminated soil in oil refinery vicinity sites was examined. The effects of pH temperature initial concentration of phenanthrene and the addition of carbon sources on biodegradation potential were also investigated. Results showed that soil samples collected from four oil refinery sites in Korea had different degrees of PAH contamination and different indigenous phenanthrene-degrading microorganisms. The optimal conditions for phenanthrene biodegradation were determined to be 30oC and pH 7.0. A significantly positive relationship was observed between the microbial growth and the rate of phenanthrene degradation. However the phenanthrene biodegradation capability of the mixed culture was not related to the degree of PAH contamination in soil. In low phenanthrene concentration the growth and biodegradation rates of the mixed cultures did not increase over those of the individual strain especially IC10. High concentration of phenanthrene inhibited the growth of microbial strains and biodegradation of phenanthrene but was less inhibitory on the mixed culture. Finally when non-ionic surfactants such as Brij 30 and Brij 35 were present at the level above critical micelle concentrations (CMCs) phenanthrene degradation was completely inhibited and delayed by the addition of Triton X100 and Triton N101.
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